May 27, 2019  
Middlesex Community College Academic Catalog 2018-19 
Middlesex Community College Academic Catalog 2018-19

BIO 255 - Molecular Biology


Course Description: An intensive lecture and laboratory course designed to provide scientific background on procedures used for cloning, isolation and characterization of a particular gene or DNA sequence in biotechnology industries. Emphasis will be placed on techniques of DNA synthesis, DNA extraction, DNA amplification (PCR), DNA sequencing, and DNA analysis through cloning, non-isotope detection systems, Southern and Northern transfers, and forensic applications of DNA fingerprinting. 3 hours lecture/3 hours laboratory
Prerequisite(s): Certificate in Biotechnology or permission of course instructor.
General Education Elective(s): Science
Note(s): N/A

Course Student Learning Outcomes:
  • Explain the structure, function and replication processes of DNA and RNA
  • Explain the role of DNA and RNA in protein synthesis
  • Perform procedures related to the use of DNA in the biotech industry
  • Calculate dilutions, standards, molarity and concentrations of solutions
  • Draw conclusions based on analysis of data gathered from experiments

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